Format

Send to

Choose Destination

Electrochemical SNP detection.

Author information

1
Department of Materials Science, Faculty of Engineering, Kyushu Institute of Technology, 1-1 Sensui-cyo, Tobata-ku, Kitakyushu-shi, Fukuoka, 804-8550, Japan.

Abstract

Detection of mismatched base on a DNA duplex, which is important for the search of the heterozygote, achieved by the comparison with the reactivity of ferrocenylcarbodiimide (1) developed by our group. Mismatched thymine base on the 30-meric DNA duplex as a model of SNPs in lipoprotein lipase (LPL) gene, could react with 1 in 20 mM borate buffer (pH 8.5) and 0.1 M NaCl containing 20% DMSO at 37 degrees C. DNA duplex modified by 1 in the mismatched thymine bases could be detected by its shifted migration time in microTAS electrophoresis.

PMID:
17150941
DOI:
10.1093/nass/nrl154
[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center