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Electrochemical detection of DNase I activity.

Author information

1
Department of Materials Science, Faculty of Engineering, Kyushu Institute of Technology, 1-1 Sensui-cyo, Tobata-ku, Kitakyushu-shi, Fukuoka 804-8550, Japan.

Abstract

DNase I in one microl of the water could quantitate electrochemically with the detection limit of 0.01 units (ca. 20 pg) by using the ferrocenyl oligonucleotide-immobilized electrode prepared by thiolated oligonucleotide and ferrocenyl carbodiimide as a simple labeling reagent of redox unit.

PMID:
17150940
DOI:
10.1093/nass/nrl153
[Indexed for MEDLINE]

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