Abstract
TRIP-Brs are a recently discovered set of proteins whose functions remain poorly characterized. Here we report the identification of TRIP-Br3 as a member of the TRIP-Br family along with evidence showing that TRIP-Brs interact with bromodomain-containing transcriptional cofactors PCAF, STAF65gamma, and KAP1. PCAF, a histone acetyltransferase; STAF65gamma, a protein associated with histone acetylation activity; and KAP1, a corepressor, influence the transcriptional activity of TRIP-Brs differentially. Finally, while all three TRIP-Brs are localized to the nucleus, TRIP-Br2 and TRIP-Br3 are also present in the cytoplasm through interaction with CRM1. Our results suggest that different TRIP-Brs function by interacting with a wide variety of bromodomain-containing transcriptional regulators in different subcellular locales.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Active Transport, Cell Nucleus / drug effects
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Binding Sites / genetics
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Blotting, Western
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Cell Cycle Proteins / genetics
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Cell Cycle Proteins / metabolism*
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Cell Line
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Cell Nucleus / metabolism*
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Cytoplasm / metabolism*
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism
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Fatty Acids, Unsaturated / pharmacology
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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HeLa Cells
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Histone Acetyltransferases / genetics
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Histone Acetyltransferases / metabolism
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Humans
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Immunoprecipitation
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Microscopy, Fluorescence
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism*
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Protein Binding
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Protein Interaction Mapping / methods
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Repressor Proteins / genetics
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Repressor Proteins / metabolism
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Trans-Activators / genetics
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Trans-Activators / metabolism*
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Transcription Factors / genetics
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Transcription Factors / metabolism
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Transcriptional Activation*
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Tripartite Motif-Containing Protein 28
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p300-CBP Transcription Factors
Substances
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CDCA4 protein, human
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Cell Cycle Proteins
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DNA-Binding Proteins
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Fatty Acids, Unsaturated
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Nuclear Proteins
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Recombinant Fusion Proteins
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Repressor Proteins
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SERTAD1 protein, human
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SERTAD2 protein, human
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Trans-Activators
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Transcription Factors
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Green Fluorescent Proteins
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Histone Acetyltransferases
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p300-CBP Transcription Factors
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p300-CBP-associated factor
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TRIM28 protein, human
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Tripartite Motif-Containing Protein 28
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leptomycin B