Transcriptional and subcellular regulation of the TRIP-Br family

Gene. 2007 Feb 15;388(1-2):102-9. doi: 10.1016/j.gene.2006.10.008. Epub 2006 Oct 24.

Abstract

TRIP-Brs are a recently discovered set of proteins whose functions remain poorly characterized. Here we report the identification of TRIP-Br3 as a member of the TRIP-Br family along with evidence showing that TRIP-Brs interact with bromodomain-containing transcriptional cofactors PCAF, STAF65gamma, and KAP1. PCAF, a histone acetyltransferase; STAF65gamma, a protein associated with histone acetylation activity; and KAP1, a corepressor, influence the transcriptional activity of TRIP-Brs differentially. Finally, while all three TRIP-Brs are localized to the nucleus, TRIP-Br2 and TRIP-Br3 are also present in the cytoplasm through interaction with CRM1. Our results suggest that different TRIP-Brs function by interacting with a wide variety of bromodomain-containing transcriptional regulators in different subcellular locales.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Active Transport, Cell Nucleus / drug effects
  • Binding Sites / genetics
  • Blotting, Western
  • Cell Cycle Proteins / genetics
  • Cell Cycle Proteins / metabolism*
  • Cell Line
  • Cell Nucleus / metabolism*
  • Cytoplasm / metabolism*
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • Fatty Acids, Unsaturated / pharmacology
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • HeLa Cells
  • Histone Acetyltransferases / genetics
  • Histone Acetyltransferases / metabolism
  • Humans
  • Immunoprecipitation
  • Microscopy, Fluorescence
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism*
  • Protein Binding
  • Protein Interaction Mapping / methods
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Repressor Proteins / genetics
  • Repressor Proteins / metabolism
  • Trans-Activators / genetics
  • Trans-Activators / metabolism*
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Transcriptional Activation*
  • Tripartite Motif-Containing Protein 28
  • p300-CBP Transcription Factors

Substances

  • CDCA4 protein, human
  • Cell Cycle Proteins
  • DNA-Binding Proteins
  • Fatty Acids, Unsaturated
  • Nuclear Proteins
  • Recombinant Fusion Proteins
  • Repressor Proteins
  • SERTAD1 protein, human
  • SERTAD2 protein, human
  • Trans-Activators
  • Transcription Factors
  • Green Fluorescent Proteins
  • Histone Acetyltransferases
  • p300-CBP Transcription Factors
  • p300-CBP-associated factor
  • TRIM28 protein, human
  • Tripartite Motif-Containing Protein 28
  • leptomycin B