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Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2007 May;103(5):610-7. Epub 2006 Nov 30.

Identification of bacteria associated with spreading odontogenic infections by 16S rRNA gene sequencing.

Author information

1
Infection and Immunity Section, University of Glasgow Dental School, Glasgow, UK. m.riggio@dental.gla.ac.uk

Abstract

OBJECTIVE:

To determine the bacterial species associated with spreading odontogenic infections (SOIs).

STUDY DESIGN:

Pus samples from 4 cases of SOI were analyzed by microbiological culture methods for the presence of bacteria, and by polymerase chain reaction (PCR) amplification, cloning, and sequencing of bacterial 16S rRNA genes.

RESULTS:

Culture methods identified species from the genera Prevotella, Streptococcus, and Fusobacterium, as well as anaerobic streptococci. Molecular detection methods identified a far more diverse microflora. The predominant genus detected was Prevotella, representing 102 (50.2%) of 203 clones analyzed. Prevotella oris was the most abundant species identified, representing 45 (22.2%) of 203 clones analyzed. Twelve clones (5.9%) represented uncultivable species, namely Prevotella PUS9.180, an uncultured Peptostreptococcus species, and an uncultured bacterium belonging to the Bacteroidetes phylum.

CONCLUSIONS:

Prevotella species may play an important role in SOIs, and further work to examine in more detail the pathogenicity determinants of these organisms and associated host responses is warranted.

PMID:
17141534
DOI:
10.1016/j.tripleo.2006.08.009
[Indexed for MEDLINE]

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