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Nat Rev Mol Cell Biol. 2006 Dec;7(12):952-8. doi: 10.1038/nrm2067.

Functional and quantitative proteomics using SILAC.

Author information

1
Department of Proteomics and Signal Transduction, Max-Planck Institute for Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany. mmann@biochem.mpg.de

Abstract

Researchers in many biological areas now routinely characterize proteins by mass spectrometry. Among the many formats for quantitative proteomics, stable-isotope labelling by amino acids in cell culture (SILAC) has emerged as a simple and powerful one. SILAC removes false positives in protein-interaction studies, reveals large-scale kinetics of proteomes and - as a quantitative phosphoproteomics technology - directly uncovers important points in the signalling pathways that control cellular decisions.

PMID:
17139335
DOI:
10.1038/nrm2067
[Indexed for MEDLINE]

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