Send to

Choose Destination
See comment in PubMed Commons below
Hum Reprod. 2007 Mar;22(3):829-35. Epub 2006 Nov 30.

Metabolism of human embryos following cryopreservation: implications for the safety and selection of embryos for transfer in clinical IVF.

Author information

  • 1Department of Biology, University of York, York, UK.

Erratum in

  • Hum Reprod. 2007 Apr;22(4):1196.



Cryopreservation of supernumerary embryos is routinely performed in human-assisted reproduction, providing a source of embryos which can be thawed for use in subsequent treatment cycles. However, the viability of cryopreserved embryos has traditionally relied on morphological assessment, which is a poor predictor of embryo health since freezing leads to a significant overall reduction in implantation potential, and its long-term efficacy is unknown. This study describes how the post-thaw metabolism of human embryos can be used to predict future development to the blastocyst stage.


HPLC was used to analyse the post-thaw amino acid metabolism of human embryos from day 2 to day 3 of development.


It was possible to predict with 87% accuracy which frozen-thawed embryo would develop to the blastocyst stage. Developmentally competent embryos were more metabolically quiescent than their arresting counterparts. Amino acid turnover was also capable of distinguishing between the developmental potential of the best, Grade I embryos P < 0.05.


The data suggests that cryopreservation in IVF is a safe procedure and that amino acid turnover can be used to select which cryopreserved embryo will develop to the blastocyst stage, irrespective of their post-thaw grade.

[PubMed - indexed for MEDLINE]
Free full text
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire
    Loading ...
    Support Center