The objective of the present investigation was to examine a proposal which stated that individual power values should be aligned according to peak power output (PPO) before calculating the mean value of PPO. This procedure removes the variation in time it takes for individuals to reach PPO. Participants were forty-one University Rugby Union Football players of mean age 21.7 +/- 2.6 years, height 181.4 +/- 6.9 cm and body mass 88.9 +/- 12.7 kg. Data were collected using a friction-belt cycle ergometer (Monark 864, Varberg, Sweden). A significantly larger mean value for PPO was found when results were calculated from time-aligned rather than cross-sectional data (1154 +/- 246 vs. 1121 +/- 254 W, p < 0.0001); the mean difference was approximately 3 %. Additionally, the average profile of the power output curve was more reflective of individual power curves. The negative correlation between PPO and the time taken to reach PPO was - 0.32 (p < 0.05), confirming the view that the earlier the time taken to reach PPO the larger the PPO. It was concluded that the mean value of PPO and the corresponding profile for power output curves are best represented by the analysis of time-aligned rather than cross-sectional data.