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Protein Sci. 2006 Dec;15(12):2842-6.

Enhanced cell-free protein expression by fusion with immunoglobulin Ckappa domain.

Author information

1
Technology Research Group, The Babraham Institute, Cambridge CB2 4AT, United Kingdom.

Abstract

While cell-free systems are increasingly used for protein expression in structural and functional studies, several proteins are difficult to express or expressed only at low levels in cell-free lysates. Here, we report that fusion of the human immunoglobulin kappa light chain constant domain (Ckappa) at the C terminus of four representative proteins dramatically improved their production in the Escherichia coli S30 system, suggesting that enhancement of cell-free protein expression by Ckappa fusion will be widely applicable.

PMID:
17132864
PMCID:
PMC2242431
DOI:
10.1110/ps.062429906
[Indexed for MEDLINE]
Free PMC Article

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