Serious bacterial infections of the eye are often associated with abiotic prosthetic materials, such as contact lenses, intraocular lenses, and scleral buckles. In recent years, microbiologists have recognized substantial differences between bacteria growing in a sessile community attached to a surface and free-living or planktonic bacteria. These sessile surface-attached communities are known as biofilms, whose properties have important consequences for clinical medicine. As a population, bacteria in biofilms are more resistant to antimicrobial agents and the immune system, and they are more persistent than planktonic bacteria in hostile environments. These characteristics are likely the result of both biofilm-specific phenotypes and increased phenotypic diversity within biofilms as compared with planktonic communities of bacteria. Bacterial biofilm formation has been observed on human tissues, as well as on abiotic prosthetic devices. A better understanding of biofilm formation may lead to the development of novel antimicrobial agents as well as prosthetic devices that are resistant to bacterial colonization.