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Drug Discov Today. 2006 Dec;11(23-24):1054-60. Epub 2006 Sep 25.

Novel fluorescent proteins for high-content screening.

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Department of Lead Discovery, Boehringer Ingelheim Pharma GmbH and Co. KG, Birkendorfer Str.65, D-88397 Biberach, Germany.


The development of fast microscopic imaging devices has enabled the application of automated fluorescence microscopy to pharmaceutical high-throughput drug-discovery assays, referred to as high-content screening (HCS). Initially, green fluorescent protein and its derivatives from Aequorea Victoria, and later anthozoan fluorescent proteins (FPs) have become potent tools as live-cell markers in HCS assays. We illustrate the broad applicability of classic and novel FPs to drug-discovery assays, giving example applications of the use of FPs in multiplexed imaging as fluorescent timers, photosensitizers and pulse-chase labels, and for robotically integrated compound testing.

[Indexed for MEDLINE]

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