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Nat Cell Biol. 2006 Dec;8(12):1327-36. Epub 2006 Nov 26.

Maintenance of colonic homeostasis by distinctive apical TLR9 signalling in intestinal epithelial cells.

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1
Department of Medicine, University of California, San Diego, La Jolla, CA 92093-0663, USA. j142l33@ucsd.edu

Abstract

The mechanisms by which commensal bacteria suppress inflammatory signalling in the gut are still unclear. Here, we present a cellular mechanism whereby the polarity of intestinal epithelial cells (IECs) has a major role in colonic homeostasis. TLR9 activation through apical and basolateral surface domains have distinct transcriptional responses, evident by NF-kappaB activation and cDNA microarray analysis. Whereas basolateral TLR9 signals IkappaBalpha degradation and activation of the NF-kappaB pathway, apical TLR9 stimulation invokes a unique response in which ubiquitinated IkappaB accumulates in the cytoplasm preventing NF-kappaB activation. Furthermore, apical TLR9 stimulation confers intracellular tolerance to subsequent TLR challenges. IECs in TLR9-deficient mice, when compared with wild-type and TLR2-deficient mice, display a lower NF-kappaB activation threshold and these mice are highly susceptible to experimental colitis. Our data provide a case for organ-specific innate immunity in which TLR expression in polarized IECs has uniquely evolved to maintain colonic homeostasis and regulate tolerance and inflammation.

PMID:
17128265
DOI:
10.1038/ncb1500
[Indexed for MEDLINE]
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