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J Immunol Methods. 2007 Jan 10;318(1-2):95-101. Epub 2006 Nov 3.

A fast and efficient procedure to produce scFvs specific for large macromolecular complexes.

Author information

1
Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA.

Abstract

We have expanded the application of antibody phage display to a new type of antigen: ribonucleoprotein (RNP) complexes. We describe a simple and efficient method for screening antibodies specific for large intact RNPs and individual components. We also describe a fast and easy method to overcome the abundance of amber stop codons in the positive phage clones. The resulting antibodies have been used in ELISA and Western blot analysis.

PMID:
17126854
PMCID:
PMC2920608
DOI:
10.1016/j.jim.2006.10.005
[Indexed for MEDLINE]
Free PMC Article

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