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Appl Environ Microbiol. 2007 Jan;73(2):659-62. Epub 2006 Nov 22.

Comparison of primer sets for use in automated ribosomal intergenic spacer analysis of aquatic bacterial communities: an ecological perspective.

Author information

1
Limnology and Marine Science Program, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.

Abstract

Two primer sets for automated ribosomal intergenic spacer analysis (ARISA) were used to assess the bacterial community composition (BCC) in Lake Mendota, Wisconsin, over 3 years. Correspondence analysis revealed differences in community profiles generated by different primer sets, but overall ecological patterns were conserved in each case. ARISA is a powerful tool for evaluating BCC change through space and time, regardless of the specific primer set used.

PMID:
17122397
PMCID:
PMC1796982
DOI:
10.1128/AEM.02130-06
[Indexed for MEDLINE]
Free PMC Article

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