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Nat Biotechnol. 2006 Dec;24(12):1569-71. Epub 2006 Nov 19.

Blue fluorescent proteins with enhanced brightness and photostability from a structurally targeted library.

Author information

1
Department of Chemical Engineering, University of California, Santa Barbara, California 93106, USA.

Abstract

The utility of blue fluorescent protein (BFP) has been limited by its low quantum yield and rapid photobleaching. A library targeting residues neighboring the chromophore yielded a variant with enhanced quantum yield (0.55 versus 0.34), reduced pH sensitivity and a 40-fold increase in photobleaching half-life. This BFP, named Azurite, is well expressed in bacterial and mammalian cells and extends the palette of fluorescent proteins that can be used for imaging.

PMID:
17115054
DOI:
10.1038/nbt1264
[Indexed for MEDLINE]

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