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J Opt Soc Am A Opt Image Sci Vis. 2006 Dec;23(12):3139-49.

On the fundamental imaging-depth limit in two-photon microscopy.

Author information

1
Max-Planck Institute for Medical Research, Heidelberg, Germany. ptheer@u.washington.edu

Abstract

We have analyzed how the maximal imaging depth of two-photon microscopy in scattering samples depends on properties of the sample and the imaging system. We find that the imaging depth increases with increasing numerical aperture and staining inhomogeneity and with decreasing excitation-pulse duration and scattering anisotropy factor, but is ultimately limited by near-surface fluorescence with slight improvements possible using special detection strategies.

PMID:
17106469
DOI:
10.1364/josaa.23.003139
[Indexed for MEDLINE]

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