Abstract
The influence of the stable prostacyclin-analogue Iloprost (Fa. Schering, Berlin) in the enzyme release from extracorporalic perfused kidneys was tested in an experimental investigation. The mechanic hypothermic perfusion was carried out on four different conditions. The estimation of specially cytosolic, lysosomal, and membrane-associated enzymes in the perfusat of the kidneys showed less activities in the Iloprost-protected kidneys. The results after the replantation of the kidneys support the interpretation of the enzyme determinations.
MeSH terms
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Acid Phosphatase / metabolism
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Amidohydrolases / metabolism
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Aminopeptidases / metabolism
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Animals
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CD13 Antigens
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Cell Membrane / enzymology
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Cold Temperature
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Cytosol / enzymology
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Enzymes / metabolism*
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Iloprost / pharmacology*
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In Vitro Techniques
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Kidney / drug effects
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Kidney / enzymology*
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Kinetics
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Leucyl Aminopeptidase / metabolism
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Lysosomes / enzymology
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Perfusion
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Regression Analysis
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Swine
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gamma-Glutamyltransferase / metabolism
Substances
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Enzymes
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gamma-Glutamyltransferase
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Acid Phosphatase
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Aminopeptidases
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Leucyl Aminopeptidase
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CD13 Antigens
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Amidohydrolases
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amidase
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Iloprost