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Neurosci Lett. 1990 Sep 18;117(3):259-63.

Plasmid DNAs directly injected into mouse brain with lipofectin can be incorporated and expressed by brain cells.

Author information

1
Faculty of Pharmaceutical Sciences, Okayama University, Japan.

Abstract

In this study, we demonstrated that lipofectin-treated DNAs which were injected into mouse brain could be incorporated and expressed by brain cells. When L7RH-beta gal plasmid DNA harboring E. coli beta-galactosidase gene fused with the nuclear location signal of SV40 T-antigen gene was injected into brains of 1-week-old mice, cells whose nuclei appeared to be densely stained with the chromogenic substrate X-gal were detected in several portions of the brain till 9 days after injection. Injection of pMLV-CAT plasmid DNA which contains the E. coli chloramphenicol acetyltransferase (CAT) gene also resulted in cells immunoreactive to the anti-CAT antibody.

PMID:
1710037
DOI:
10.1016/0304-3940(90)90673-w
[Indexed for MEDLINE]

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