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Nat Methods. 2006 Dec;3(12):977-9. Epub 2006 Nov 12.

A highly sensitive protein-protein interaction assay based on Gaussia luciferase.

Author information

1
Département de Biochimie, Université de Montréal, C.P. 6128, Succursale centre-ville, Montréal, Québec, H3C 3J7, Canada.

Abstract

Protein-fragment complementation assays (PCAs) provide a general strategy to study the dynamics of protein-protein interactions in vivo and in vitro. The full potential of PCA requires assays that are fully reversible and sensitive at subendogenous protein expression levels. We describe a new assay that meets these criteria, based on the Gaussia princeps luciferase enzyme, demonstrating chemical reversal, and induction and inhibition of a key interaction linking insulin and TGFbeta signaling.

PMID:
17099704
DOI:
10.1038/nmeth979
[Indexed for MEDLINE]

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