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Proc Natl Acad Sci U S A. 2006 Nov 21;103(47):17813-7. Epub 2006 Nov 8.

An efficient promoter trap for detection of patterned gene expression and subsequent functional analysis in Drosophila.

Author information

1
University of California, Life Sciences Building 4214, Los Angeles, CA 90025, USA.

Abstract

Transposable elements have been used in Drosophila to detect gene expression, inactivate gene function, and induce ectopic expression or overexpression. We have combined all of these features in a single construct. A promoterless GAL4 cDNA is expressed when the construct inserts within a transcriptional unit, and GAL4 activates a GFP-encoding gene present in the same transposon. In a primary screen, patterned gene expression is detected as GFP fluorescence in the live progeny of dysgenic males. Many animals expressing GFP in distinct patterns can be recovered with relatively little effort. As expected, many insertions cause loss of function. After insertion at a genomic location, specific parts of the transposon can be excised by FLP recombinase, thus allowing it to induce conditional misexpression of the tagged gene. Therefore, both gain- and loss-of-function studies can be carried out with a single insertion in a gene identified by virtue of its expression pattern. Using this promoter trap approach, we have identified a group of cells that innervate the calyx of the mushroom body and could thus define a previously unrecognized memory circuit.

PMID:
17093046
PMCID:
PMC1635159
DOI:
10.1073/pnas.0607652103
[Indexed for MEDLINE]
Free PMC Article

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