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Exp Lung Res. 2006 Sep;32(8):349-62.

Inhibition of protein kinase C epsilon causes ciliated bovine bronchial cell detachment.

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1
Pulmonary, Critical Care and Sleep Medicine Section, Department of Internal Medicine, University of Nebraska Medical Center, Omaha, Nebraska 68198-5300, USA.

Abstract

This study defines the in vitro phenomenon of ciliated bovine bronchial epithelial cell (BBEC) detachment from the basal epithelium and regulation of cilia motility mediated through protein kinase C epsilon (PKCepsilon). The authors determined the time course of activation and downregulation of PKCepsilon by the known PKC activator phorbol 12-myristate 13-acetate (PMA) and demonstrate that chemical inhibition of PKC by calphostin C or the novel PKC isoform inhibitor Ro 31-8220 induced striking detachment of ciliated BBECs from the basal cell monolayer within 1 hour, independent of apoptosis or necrotic cell death. The results of this study support a possible novel PKCepsilon-mediated signaling pathway through which ciliated cell attachment is maintained.

PMID:
17090476
PMCID:
PMC2100410
DOI:
10.1080/01902140600959630
[Indexed for MEDLINE]
Free PMC Article
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