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Antimicrob Agents Chemother. 2007 Jan;51(1):350-3. Epub 2006 Nov 6.

Simple colorimetric inhibition assay of heme crystallization for high-throughput screening of antimalarial compounds.

Author information

1
Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.

Abstract

Current assays for screening new antimalarials need initiators of beta-hematin formation that require laborious preparation, special devices, and substrates. In this study, based on reduction of heme absorption in beta-hematin formation, we developed a simple colorimetric assay using Tween 20 as an initiator and a microplate reader for high-throughput screening of inhibitors of beta-hematin formation.

PMID:
17088494
PMCID:
PMC1797674
DOI:
10.1128/AAC.00985-06
[Indexed for MEDLINE]
Free PMC Article

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