(A) Consensus labeling pattern showing the overlap of Cha-Gal4 expression (detected with UAS-EGFP) with NCCAP (detected by anti-CCAP immunolabeling; n=9). Circles, CCAP-immunopositive neurons. Green circles, EGFP labeled in greater than one-third of the preparations. Light blue circles, EGFP labeled in one-third or less of the preparations. Gray circles, neurons that were never EGFP-labeled. Filled circles, consistently high EGFP expression (see Experimental Procedures). Open circles, low EGFP expression. In paired NCCAP neurons in hemisegments T3–A4, one was typically strongly CCAP-immunopositive and the other was more weakly labeled. For convenience, we have shown the strongly expressing neuron as medial to the weakly expressing one. This does not necessarily represent their anatomical positions.
(B–J) High-resolution confocal micrographs showing anti-CCAP immunoreactivity (B, E, H), EGFP expression (C, F, I), and their overlap (D, G, J). For each set of images, individual neurons are indicated by arrows and dotted outlines. Boxes in (A) show the anatomical location of the corresponding images. In hemisegments T3 and A1 to A4, only the more weakly CCAP immunoreactive neuron of the pair expresses EGFP.