Format

Send to

Choose Destination
Neuron. 2006 Nov 9;52(3):425-36.

Refined spatial manipulation of neuronal function by combinatorial restriction of transgene expression.

Author information

1
Laboratory of Molecular Biology, National Institute of Mental Health, 9000 Rockville Pike, Bethesda, Maryland 20892, USA.

Abstract

Selective genetic manipulation of neuronal function in vivo requires techniques for targeting gene expression to specific cells. Existing systems accomplish this using the promoters of endogenous genes to drive expression of transgenes directly in cells of interest or, in "binary" systems, to drive expression of a transcription factor or recombinase that subsequently activates the expression of other transgenes. All such techniques are constrained by the limited specificity of the available promoters. We introduce here a combinatorial system in which the DNA-binding (DBD) and transcription-activation (AD) domains of a transcription factor are independently targeted using two different promoters. The domains heterodimerize to become transcriptionally competent and thus drive transgene expression only at the intersection of the expression patterns of the two promoters. We use this system to dissect a neuronal network in Drosophila by selectively targeting expression of the cell death gene reaper to subsets of neurons within the network.

PMID:
17088209
PMCID:
PMC1713190
DOI:
10.1016/j.neuron.2006.08.028
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center