Send to

Choose Destination
Nat Genet. 2006 Dec;38(12):1406-12. Epub 2006 Nov 5.

Comparative genome sequencing of Escherichia coli allows observation of bacterial evolution on a laboratory timescale.

Author information

Department of Bioengineering, University of California, San Diego, California 92093, USA.


We applied whole-genome resequencing of Escherichia coli to monitor the acquisition and fixation of mutations that conveyed a selective growth advantage during adaptation to a glycerol-based growth medium. We identified 13 different de novo mutations in five different E. coli strains and monitored their fixation over a 44-d period of adaptation. We obtained proof that the observed spontaneous mutations were responsible for improved fitness by creating single, double and triple site-directed mutants that had growth rates matching those of the evolved strains. The success of this new genome-scale approach indicates that real-time evolution studies will now be practical in a wide variety of contexts.

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Nature Publishing Group
Loading ...
Support Center