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Biochem Biophys Res Commun. 2006 Dec 22;351(3):756-63. Epub 2006 Oct 30.

A PCR-based method for detection and quantification of small RNAs.

Author information

1
Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, NV 89557, USA.

Abstract

Recent cloning efforts have identified hundreds of thousands of small RNAs including micro RNAs (miRNAs), Piwi-interacting RNAs (piRNAs), and small nucleolar RNAs (snoRNAs). These non-coding small RNAs need to be further validated and characterized by detecting and quantifying their expression in different tissues and during different developmental courses. A simple, accurate, and sensitive method for small RNA expression profiling is in high demand. Here, we report such a PCR-based method.

PMID:
17084816
PMCID:
PMC1934510
DOI:
10.1016/j.bbrc.2006.10.105
[Indexed for MEDLINE]
Free PMC Article

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