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Anaerobe. 2006 Oct-Dec;12(5-6):279-82.

Degradation of human hemoglobin by Prevotella intermedia.

Author information

1
Department of Oral Biology, School of Stomatology, Fourth Military Medical University, 145 West Chang Le Road, Xi'an 710032, PR China. jchsmg@fmmu.edu.cn

Abstract

In this study, the ability of Prevotella intermedia, an obligate anaerobic rod, to degrade human hemoglobin was determined by SDS-PAGE and the degradation was quantified by scanning densitometry. Both bacterial cells and culture supernatants degraded hemoglobin. The hemoglobin degradation by P. intermedia was time-dependent, heat sensitive, pH related and was not influenced by iron restriction. Inhibition studies demonstrated that a cysteine protease might be involved in hemoglobin degradation and this protease might require metal ions for its activity and it might be thiol-requiring and trypsin-inducible. The results indicate that P. intermedia is capable to release heme from hemoglobin, hence provide a source of iron for its proliferation.

PMID:
17081784
DOI:
10.1016/j.anaerobe.2006.09.001
[Indexed for MEDLINE]

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