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Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Nov 1;62(Pt 11):1138-40. Epub 2006 Oct 20.

Expression, purification and crystallization of a PCI domain from the COP9 signalosome subunit 7 (CSN7).

Author information

1
Department of Biochemistry, Faculty of Life Sciences, Tel Aviv University, Ramat Aviv 69978, Israel.

Abstract

A core fragment of Arabidopsis thaliana COP9 signalosome (CSN) subunit 7 was expressed in Escherichia coli. The protein was purified to homogeneity and screened for crystallization. Crystallization conditions were refined using the sitting-drop vapour-diffusion method. Crystals were obtained using polyethylene glycol 8000 as a precipitant and have a thick rod-like morphology. Their crystallographic symmetry is orthorhombic, space group C222(1), with unit-cell parameters a = 57.2, b = 86.2, c = 72.6 A and a diffraction limit of 2.06 A.

PMID:
17077498
PMCID:
PMC2225213
DOI:
10.1107/S1744309106041959
[Indexed for MEDLINE]
Free PMC Article

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