Format

Send to

Choose Destination
J Am Soc Mass Spectrom. 2007 Feb;18(2):234-44. Epub 2006 Oct 30.

Electron detachment dissociation of glycosaminoglycan tetrasaccharides.

Author information

1
Department of Chemistry, University of Georgia, Athens, Georgia 30602, USA.

Abstract

The first application of electron detachment dissociation (EDD) to carbohydrates is presented. The structural characterization of glycosaminoglycan (GAG) oligosaccharides by mass spectrometry is a longstanding problem because of the lability of these acidic, polysulfated carbohydrates. Doubly-charged negative ions of four GAG tetrasaccharides are examined by EDD, collisionally activated dissociation (CAD), and infrared multiphoton dissociation (IRMPD). EDD is found to produce information-rich mass spectra with both cross ring and glycosidic cleavage product ions. In contrast, most of the product ions produced by CAD and IRMPD result from glycosidic cleavage. EDD shows great potential as a tool for locating the sites of sulfation and other modifications in glycosaminoglycan oligosaccharides.

PMID:
17074503
PMCID:
PMC1784114
DOI:
10.1016/j.jasms.2006.09.020
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for PubMed Central
Loading ...
Support Center