Format

Send to

Choose Destination
Microb Ecol. 2007 Apr;53(3):435-42. Epub 2006 Oct 28.

Methodological considerations for the use of stable isotope probing in microbial ecology.

Author information

1
Department of Biological Sciences, University of Warwick, Coventry, CV4 7AL, UK.

Abstract

Stable isotope probing (SIP) is a method used for labeling uncultivated microorganisms in environmental samples or directly in field studies using substrate enriched with stable isotope (e.g., (13)C). After consumption of the substrate, the cells of microorganisms that consumed the substrate become enriched in the isotope. Labeled biomarkers, such as phospholipid-derived fatty acid (PLFA), ribosomal RNA, and DNA can be analyzed with a range of molecular and analytical techniques, and used to identify and characterize the organisms that incorporated the substrate. The advantages and disadvantages of PLFA-SIP, RNA-SIP, and DNA-SIP are presented. Using examples from our laboratory and from the literature, we discuss important methodological considerations for a successful SIP experiment.

PMID:
17072677
DOI:
10.1007/s00248-006-9125-x
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center