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Methods Mol Biol. 2006;347:237-52.

Micromethods for the characterization of lipid A-core and O-antigen lipopolysaccharide.

Author information

1
Department of Microbiology and Immunology, University of Western Ontario, London, Ontario, Canada.

Abstract

Methods for rapid and simple analysis of lipopolysaccharide (LPS) from bacterial whole-cell lysates or membrane preparations have contributed to advancing our knowledge of the genetics of the LPS biogenesis. LPS, a major constituent of the outer membranes in Gram-negative bacteria, has a complex mechanism of synthesis and assembly that requires the coordinated participation of many genes and gene products. This chapter describes a collection of methods routinely used in our laboratory for the characterization of LPS in Escherichia coli and other bacteria.

PMID:
17072014
DOI:
10.1385/1-59745-167-3:237
[Indexed for MEDLINE]

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