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Virology. 2007 Mar 15;359(2):459-65. Epub 2006 Oct 30.

The NSm proteins of Rift Valley fever virus are dispensable for maturation, replication and infection.

Author information

1
Department of Epidemiology, School of Public Health, University of Michigan, 109 S. Observatory, Ann Arbor, MI 48109, USA. gerrard@umich.edu

Abstract

Rift Valley fever (RVF) virus belongs to the Bunyaviridae family of segmented negative-strand RNA viruses and causes mosquito-borne disease in sub-Saharan Africa. We report the development of a T7 RNA polymerase-driven plasmid-based genetic system for the virulent Egyptian isolate, ZH501. We have used this system to rescue a virus that has a 387 nucleotide deletion on the genomic M segment that eliminates the coding region for two non-structural proteins known as NSm. This virus, DeltaNSm rZH501, is indistinguishable from the parental ZH501 strain with respect to expression of structural proteins and growth in cultured mammalian cells.

PMID:
17070883
PMCID:
PMC2364454
DOI:
10.1016/j.virol.2006.09.035
[Indexed for MEDLINE]
Free PMC Article

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