Format

Send to

Choose Destination
J Comp Neurol. 1990 Dec 22;302(4):729-38.

Long-term survival and sprouting in culture by motoneurons isolated from the spinal cord of adult frogs.

Author information

1
Department of Pharmacology, Biocenter of the University, Basel, Switzerland.

Abstract

Motoneurons of adult frog spinal cord have been retrogradely labeled with the carbocyanine derivative diI. Spinal cords were then dissociated and the labeled motoneurons partially purified by centrifugation over a bovine serum albumin (BSA) density gradient. The resulting cell suspension was plated on a substrate of innervated muscle extracellular matrix (ECM) to which the motoneurons attached and extended processes. Labeled adult motoneurons survived for more than 4 weeks in a defined medium in the absence of added serum or growth factors. These cultures of adult motoneurons provide a favorable preparation for studying molecular factors that influence process outgrowth and synapse formation.

PMID:
1707066
DOI:
10.1002/cne.903020405
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center