Format

Send to

Choose Destination
Nucleic Acids Res. 2007 Jan;35(Database issue):D805-9. Epub 2006 Oct 26.

qPrimerDepot: a primer database for quantitative real time PCR.

Author information

1
Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, Bethesda, MD 20892-4605.

Abstract

Gene expression studies employing high throughput real time PCR methods require finding uniform conditions for optimal amplification of multiple targets, often a daunting task. We developed a primer database, qPrimerDepot, which provides optimized primers for all human and mouse RefSeq genes. These primers are designed to amplify desired templates under unified annealing temperature. For most intron-bearing genes, primers flank one of the largest introns thus minimizing background noise due to genomic DNA contamination. The qPrimerDepot database can be accessed at http://primerdepot.nci.nih.gov/ and http://mouseprimerdepot.nci.nih.gov/.

PMID:
17068075
PMCID:
PMC1635330
DOI:
10.1093/nar/gkl767
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Silverchair Information Systems Icon for PubMed Central
Loading ...
Support Center