Format

Send to

Choose Destination
J Mol Diagn. 2006 Nov;8(5):544-50.

Betaine, dimethyl sulfoxide, and 7-deaza-dGTP, a powerful mixture for amplification of GC-rich DNA sequences.

Author information

1
Istituto Giannina Gaslini, Lab Genetica Molecolare, L. go G. Gaslini 5, 16148 Genova, Italy.

Abstract

Currently, polymerase chain reaction is the most used technique in many laboratories for either diagnostic or molecular biology purposes. Despite the large number of DNA sequences that can be easily analyzed, some GC-rich sequences are refractory to amplification due to the formation of secondary intramolecular structures. To overcome this problem, several molecules have been described to improve polymerization. Here we show that a combination of three additives--betaine, dimethyl sulfoxide, and 7-deaza-dGTP--was essential to achieve amplification of DNA sequences of three disease genes showing a GC content ranging from 67 to 79%.

PMID:
17065422
PMCID:
PMC1876170
DOI:
10.2353/jmoldx.2006.060058
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center