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Nat Methods. 2006 Nov;3(11):887-9.

Targeting and tracing antigens in live cells with fluorescent nanobodies.

Author information

1
Ludwig Maximilians University Munich, Department of Biology II, Grosshaderner Str. 2, 82152 Planegg-Martinsried, Germany.

Abstract

We fused the epitope-recognizing fragment of heavy-chain antibodies from Camelidae sp. with fluorescent proteins to generate fluorescent, antigen-binding nanobodies (chromobodies) that can be expressed in living cells. We demonstrate that chromobodies can recognize and trace antigens in different subcellular compartments throughout S phase and mitosis. Chromobodies should enable new functional studies, as potentially any antigenic structure can be targeted and traced in living cells in this fashion.

PMID:
17060912
DOI:
10.1038/nmeth953
[Indexed for MEDLINE]
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