Format

Send to

Choose Destination
J Microbiol Methods. 2007 Feb;68(2):434-6. Epub 2006 Oct 19.

Rapid isolation of rare clones from highly complex DNA libraries by PCR analysis of liquid gel pools.

Author information

1
Harvard College, Cambridge, USA.

Abstract

A semiliquid medium was employed in an efficient method to screen highly complex DNA libraries for clones with known sequences. Unbiased clone pools are generated in 2 ml vials and screened by whole-cell PCR, and individual clones are obtained by few additional rounds of dilution and PCR screening. To demonstrate the utility of this approach, the single positive clone present in a 400,000 member metagenomic fosmid library was isolated.

PMID:
17055603
DOI:
10.1016/j.mimet.2006.09.009
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center