Send to

Choose Destination
Acta Pharmacol Sin. 2006 Nov;27(11):1509-14.

Expression of nicotinic acetylcholine receptor subunit alpha9 in type II vestibular hair cells of rats.

Author information

Department of Otorhinolaryngology, Union Hospital of Tongji Medical College, Huazhong Science and Technology University, Wuhan 430030, China.



To explore the cell specific existence of alpha 9 AChR in the vestibular type II hair cells (VHC II) of rats.


To detect the expression of alpha 9 AChR messenger RNA (mRNA) in the vestibular endorgans and single VHC II of rats by using the reverse transcription polymerase chain reaction (RT-PCR) technique and the single cell RT-PCR technique, respectively.


It was shown that alpha 9 AChR mRNA was detected in the vestibular endorgans. By using single-cell RT-PCR, mRNA encoding alpha 9 AChR was also detected in the VHC II of the rats. Sequence analysis of the PCR products confirmed identity to corresponding cDNA sequence in the predicted region.


We established a method which could effectively detect the cell specific expression of mRNA in an individual VHC. Present data confirm that alpha 9 AChR mRNA is expressed in the VHC II of rats and indicates that alpha 9 AChR may function as a mediator of efferent cholinergic signaling in mammalian VHC.

[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Nature Publishing Group
Loading ...
Support Center