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Diabetologia. 2006 Nov;49(11):2704-12. Epub 2006 Sep 20.

Beta cell death in hyperglycaemic Psammomys obesus is not cytokine-mediated.

Author information

1
Centre of Anatomy and Institute of Clinical Biochemistry, Hannover Medical School, 30625 Hannover, Germany. joerns.anne@mh-hannover.de

Abstract

AIMS/HYPOTHESIS:

It has recently been proposed that IL-1beta may be responsible for beta cell death in type 2 diabetes mellitus. Major support for this assumption was derived from experiments in the gerbil Psammomys obesus (sand rat), a model for nutritionally induced non-insulin-dependent type 2 diabetes. Using gerbil-specific primers for the analysis of gene expression, we investigated the validity of this hypothesis.

METHODS:

Gene expression was analysed by real-time RT-PCR of isolated and laser-microdissected islets and by in situ RT-PCR, both in beta cells and in immune cells, as well as in lymph nodes and spleen.

RESULTS:

We were unable to detect Il-1beta and the IL-1beta-inducible enzyme inducible nitric oxide synthase (iNos) by in situ RT-PCR, either in the pancreatic beta cells, or in the small number of non-activated immune cells of healthy and diabetic Psammomys obesus after 1 and 3 weeks on a high-energy diet. Very low levels of Il-1beta and iNos mRNA were detectable in collagenase-isolated and laser-microdissected islets of normoglycaemic gerbils by real-time RT-PCR without any increase of these mRNAs in islets from diabetic animals. These results were confirmed by electron microscopy with immunogold staining for IL-1beta and insulin.

CONCLUSIONS/INTERPRETATION:

The diabetic syndrome induced in Psammomys obesus by high-energy diet is a classical type 2 diabetes model, which does not show any evidence of an involvement of the proinflammatory cytokine IL-1beta or of activated immune cells in its pathogenesis. This is clearly at variance with the situation in type 1 diabetes.

PMID:
17047921
DOI:
10.1007/s00125-006-0413-2
[Indexed for MEDLINE]

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