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J Membr Biol. 1990 Oct;118(1):69-75.

Chloride channels in human platelets: evidence for activation by internal calcium.

Author information

1
Physiological Laboratory, Cambridge, England.

Abstract

Whole-cell patch-clamp recordings were made from freshly isolated human platelets. The pipette contained a high concentration of divalent cations, which permitted easy disruption of cell-attached membrane patches by suction. Single-channel currents were measured when the pipette contained isotonic BaCl2 or MgCl2 saline; over 30 sec -5 min an increasing number of channels appeared until conductance steps through individual channels could no longer be distinguished. The current-voltage relationship was curvilinear; chord conductance at -35 mV was 25 pS increasing to 45 to 52 pS at +45 mV. Ion substitution experiments showed the current to be primarily carried by Cl-. Erev was shifted 30 mV/10-fold change in external Cl- (replaced by gluconate), was similar with BaCl2 or MgCl2 in the pipette and was not significantly shifted by replacing external Na+ with K+. Addition of 1 mM BAPTA to the MgCl2 pipette saline prevented activation of Cl- currents; with isotonic CaCl2 internal saline, current appeared immediately upon patch rupture, suggesting that the Cl- channels are dependent on internal Ca2+. 5-nitro-2-(3-phenylpropylamino)-benzoate, reported to block a Cl- conductance in studies of rat epithelial cells, caused a potent flickery block and may be a useful tool with which to investigate the physiological role of Cl- currents in human platelets.

PMID:
1704439
DOI:
10.1007/bf01872205
[Indexed for MEDLINE]

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