Format

Send to

Choose Destination
See comment in PubMed Commons below
Methods Mol Biol. 2007;352:95-109.

Monobodies: antibody mimics based on the scaffold of the fibronectin type III domain.

Author information

1
Department of Biochemistry and Molecular Biology, The University of Chicago, IL, USA.

Abstract

We developed the use of the 10th fibronectin type III domain of human fibronectin (FNfn10) as a scaffold to display multiple surface loops for target binding. We termed FNfn10 variants with novel binding function "monobodies." FNfn10 is a small (94 residues) protein with a beta-sandwich structure similar to the immunoglobulin fold. It is highly stable without disulfide bonds or metal ions, and it can be expressed in the correctly folded form at a high level in bacteria. These desirable physical properties render the FNfn10 scaffold compatible with virtually any display technologies. This chapter describes methods for library construction and screening and for the production of monobodies.

PMID:
17041261
DOI:
10.1385/1-59745-187-8:95
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Springer
    Loading ...
    Support Center