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Proc Natl Acad Sci U S A. 2006 Oct 24;103(43):15830-4. Epub 2006 Oct 12.

Structural basis for mRNA and tRNA positioning on the ribosome.

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1
Department of Molecular and Cell Biology, and Chemistry, University of California, Berkeley, CA 94720, USA.

Erratum in

  • Proc Natl Acad Sci U S A. 2006 Dec 26;103(52):19931. Cate, Jamie H Doudna [corrected to Cate, Jamie H D].

Abstract

Protein synthesis requires the accurate positioning of mRNA and tRNA in the peptidyl-tRNA site of the ribosome. Here we describe x-ray crystal structures of the intact bacterial ribosome from Escherichia coli in a complex with mRNA and the anticodon stem-loop of P-site tRNA. At 3.5-A resolution, these structures reveal rearrangements in the intact ribosome that clamp P-site tRNA and mRNA on the small ribosomal subunit. Binding of the anticodon stem-loop of P-site tRNA to the ribosome is sufficient to lock the head of the small ribosomal subunit in a single conformation, thereby preventing movement of mRNA and tRNA before mRNA decoding.

PMID:
17038497
PMCID:
PMC1635088
DOI:
10.1073/pnas.0607541103
[Indexed for MEDLINE]
Free PMC Article
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