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[Evidence for the presence of motilin receptor and a study on the mechanism of motilin induced Ca2+ signaling in rat myenteric neurons].

[Article in Chinese]

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Department of Gastroenterology, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, China.



To observe whether the motilin receptor (MTLR) can be expressed in primarily cultured myenteric neurons of rats and investigate the mechanism of motilin induced Ca2+ signaling in myenteric neurons of rats.


Expression of the motilin receptor was identified with double-immunofluorescence staining technique. Data on the intracellular Ca2+ concentration ([Ca2+]i) of cultured myenteric neurons with different treatments were collected by measuring Ca2+ fluorescent intensity (FI) in each neuron under confocal microscope.


The cultured myenteric neurons showed positive motilin receptor immunoreactivity. In Hank's solution, 10(-6) mol/L motilin could elevate [Ca2+]i, its height of peak being 30.6 +/- 3.7 and its FI relative change percentage being (100. 8 +/- 18.4)%. In D-Hank's solution (after removal of extracellular Ca2+, or after treatment with verapamil,an L-type calcium channel blocker), motilin could induce a small increase of [Ca2+]i. After pretreatment with NEM,a G protein inhibitor, and Compound 48/80, a PLC inhibitor, in Hank's solution respectively, motilin was inhibited and the [Ca2+]i was significantly lower than that of the group to which was added only motilin (P < 0.05). After pretreatment with D-sphingosine, a PKC inhibitor, the effect of motilin was not significantly different from that of the group to which was added only motilin (P > 0.05).


The motilin receptor could be expressed by cultured myenteric neurons of rats. Motilin could increase [Ca+]i. The increase of [Ca2+]i was caused by release of intracellular stores and influx of extracellular Ca2+, mainly through the L-type calcium channel. The motilin receptor-coupled G-protein, PLC and IP3 pathway participated in the release of Ca2+ from intracellular stores.

[Indexed for MEDLINE]

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