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J Allergy Clin Immunol. 2006 Oct;118(4):915-22. Epub 2006 Jul 24.

Cloning and characterization of profilin (Pru du 4), a cross-reactive almond (Prunus dulcis) allergen.

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  • 1Department of Biological Science, Florida State University, FL 32306-4370, USA.

Abstract

BACKGROUND:

The identity of allergenic almond proteins is incomplete.

OBJECTIVE:

Our objective was to characterize patient IgE reactivity to a recombinant and corresponding native almond allergen.

METHODS:

An almond cDNA library was screened with sera from patients with allergy for IgE binding proteins. Two reactive clones were sequenced, and 1 was expressed. The expressed recombinant allergen and its native counterpart (purified from unprocessed almond flour) were assayed by 1-dimensional and 2-dimensional gel electrophoresis, dot blot, and ELISA, and screened for cross-reactivity with grass profilin.

RESULTS:

The 2 selected clones encoded profilin (designated Pru du 4) sequences that differed by 2 silent mutations. By dot-blot analyses, 6 of 18 patient sera (33%) reacted with the recombinant Pru du 4 protein, and 8 of 18 (44%) reacted with the native form. ELISA results were similar. Almond and ryegrass profilins were mutually inhibitable. Two-dimensional immunoblotting revealed the presence of more than 1 native almond profilin isoform. The strength of reactivity of some patients' serum IgE differed markedly between assays and between native and recombinant profilins.

CONCLUSION:

Almond nut profilin is an IgE-binding food protein that is cross-reactive with grass pollen profilin and is susceptible to denaturation, resulting in variable reactivity between assay types and between patients.

CLINICAL IMPLICATIONS:

Serum IgE of nearly half of the tested patients with almond allergy reacts with almond nut profilin. Because most patients also had pollinosis, the well-known cross-reactivity between pollen and food profilins could account for this pattern of reactivity.

PMID:
17030246
DOI:
10.1016/j.jaci.2006.05.028
[PubMed - indexed for MEDLINE]
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