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J Clin Microbiol. 2006 Oct;44(10):3778-80.

Identification of Borrelia burgdorferi ribosomal protein L25 by the phage surface display method and evaluation of the protein's value for serodiagnosis.

Author information

1
University of Konstanz, Biochemical Pharmacology, 78457 Konstanz, Germany.

Abstract

The phage surface display technique was used to identify Borrelia burgdorferi antigens. By affinity selection with immunoglobulin G from pooled sera of six Lyme borreliosis (LB) patients, the ribosomal protein L25 was identified. The diagnostic value of L25 was investigated by an enzyme-linked immunosorbent assay, using sera from 80 LB patients and 75 controls, and the use of the protein resulted in a specificity of 99% and a 23% sensitivity, which qualify L25 as a useful antigen when combined with others.

PMID:
17021109
PMCID:
PMC1594769
DOI:
10.1128/JCM.00371-06
[Indexed for MEDLINE]
Free PMC Article

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