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Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Oct 1;62(Pt 10):1024-6. Epub 2006 Sep 30.

Crystallization and preliminary X-ray diffraction analysis of Salmonella typhi PilS.

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1
Department of Biological Sciences, National University of Singapore, Singapore 117543, Singapore.

Abstract

The structure determination of PilS, a type IV pilin, by X-ray crystallography is reported. The recombinant protein from Salmonella typhi was overexpressed, purified and crystallized. The crystals belong to space group P2(1)2(1)2, with unit-cell parameters a = 77.88, b = 114.53, c = 31.75 A. The selenomethionine derivative of the PilS protein was overexpressed, purified and crystallized in the same space group. Data sets have been collected to 2.1 A resolution from the selenomethionine-derivative crystal using synchrotron radiation for multiwavelength anomalous dispersion (MAD) phasing.

PMID:
17012803
PMCID:
PMC2225195
DOI:
10.1107/S174430910603661X
[Indexed for MEDLINE]
Free PMC Article

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