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Methods. 2006 Oct;40(2):135-42.

Proteomic identification of palmitoylated proteins.

Author information

1
Department of Pharmacology, Wayne State University School of Medicine, Elliman Building, Room 1224, 421 E. Canfield, Detroit, MI 48201, USA.

Abstract

A proteomic method that purifies and identifies palmitoylated proteins from complex protein extracts is described. Using the fatty acid exchange labeling chemistry (described in the preceding report), palmitoyl modifications are exchanged for biotinylated compounds, allowing the subset of palmitoyl-proteins to be affinity-purified and then identified by mass spectroscopic protein identification technologies. The advantages and pitfalls of this new technology are discussed within the context of the recent application of this method in the yeast Saccharomyces cerevisiae.

PMID:
17012025
PMCID:
PMC2806795
DOI:
10.1016/j.ymeth.2006.05.026
[Indexed for MEDLINE]
Free PMC Article

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