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Biotechnol Lett. 2006 Nov;28(21):1713-8. Epub 2006 Aug 3.

Construction and characterization of a novel maltose inducible expression vector in Bacillus subtilis.

Author information

1
Institute of Biochemical Engineering, College of Material Science and Chemical Engineering, Zhejiang University, Hangzhou 310027, P.R. China.

Abstract

A maltose-inducible expression vector in Bacillus subtilis has been developed and characterized. The vector permitted beta-galactosidase expression at a high level (maximum activity, 8.16 U/ml) when induced and its expression was markedly repressed by glucose. Using this vector, we successfully expressed the other two genes, bioA and vgb. This thus provided a potential expression system for cloned genes in B. subtilis.

PMID:
17001500
DOI:
10.1007/s10529-006-9146-z
[Indexed for MEDLINE]

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