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J Biol Chem. 2006 Nov 24;281(47):35735-41. Epub 2006 Sep 21.

The potassium channel KAT1 is activated by plant and animal 14-3-3 proteins.

Author information

1
Dipartimento di Biologia and Istituto di Biofisica-Consiglio Nazionale delle Ricerche, Università degli Studi di Milano, 20133 Milano, Italy.

Abstract

14-3-3 proteins modulate the plant inward rectifier K+ channel KAT1 heterologously expressed in Xenopus oocytes. Injection of recombinant plant 14-3-3 proteins into oocytes shifted the activation curve of KAT1 by +11 mV and increased the tau(on). KAT1 was also modulated by 14-3-3 proteins of Xenopus oocytes. Titration of the endogenous 14-3-3 proteins by injection of the peptide Raf 621p resulted in a strong decrease in KAT1 current (approximately 70% at -150 mV). The mutation K56E performed on plant protein 14-3-3 in a highly conserved recognition site prevented channel activation. Because the maximal conductance of KAT1 was unaffected by 14-3-3, we can exclude that they act by increasing the number of channels, thus ruling out any effect of these proteins on channel trafficking and/or insertion into the oocyte membrane. 14-3-3 proteins also increased KAT1 current in inside-out patches, suggesting a direct interaction with the channel. Direct interaction was confirmed by overlay experiments with radioactive 14-3-3 on oocyte membranes expressing KAT1.

PMID:
16990282
DOI:
10.1074/jbc.M603361200
[Indexed for MEDLINE]
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