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Methods Mol Biol. 2006;343:43-53.

Three methods for the introduction of foreign DNA into Agrobacterium.

Author information

1
Plant Science Institute, Department of Biology, University of Pennsylvania, PA, USA.

Abstract

The genetic manipulation of Agrobacterium tumefaciens is used to facilitate studies of bacterial gene functions or as a first step in introducing genetic material into transformable plant cells through the use of T-DNA binary vectors. Three methods are commonly used. Transformation with purified plasmid can be done with either electroporation or a simple freeze/thaw transformation method. Alternatively, a mobilizable plasmid can be placed into Agrobacterium using the triparental mating method. Here we present three detailed protocols for Agrobacterium strain construction using electroporation, the freeze/thaw method of transformation, and triparental mating.

PMID:
16988332
DOI:
10.1385/1-59745-130-4:43
[Indexed for MEDLINE]

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