In vitro selection of RNA molecules that bind specific ligands

Nature. 1990 Aug 30;346(6287):818-22. doi: 10.1038/346818a0.

Abstract

Subpopulations of RNA molecules that bind specifically to a variety of organic dyes have been isolated from a population of random sequence RNA molecules. Roughly one in 10(10) random sequence RNA molecules folds in such a way as to create a specific binding site for small ligands.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Binding Sites
  • Chromatography, Affinity
  • Cloning, Molecular
  • Coloring Agents / metabolism*
  • DNA / chemical synthesis
  • Ligands
  • Molecular Sequence Data
  • Molecular Structure
  • Nucleic Acid Conformation
  • Polymerase Chain Reaction
  • RNA / biosynthesis
  • RNA / isolation & purification*
  • RNA / metabolism
  • Templates, Genetic
  • Transcription, Genetic
  • Triazines / metabolism

Substances

  • Coloring Agents
  • Ligands
  • Triazines
  • procion blue MX-R
  • Cibacron Blue F 3GA
  • RNA
  • DNA