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Curr Opin Neurobiol. 2006 Oct;16(5):551-61. Epub 2006 Sep 12.

Imaging spatiotemporal dynamics of neuronal signaling using fluorescence resonance energy transfer and fluorescence lifetime imaging microscopy.

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Neurobiology Department, Duke University Medical Center, Research Drive, Durham, NC 27710 USA.

Erratum in

  • Curr Opin Neurobiol. 2007 Feb;17(1):128.


The spatiotemporal localization of neuronal signaling is important for triggering neuronal responses in specific locations at precise times. Fluorescence resonance energy transfer imaging enables measurement of spatiotemporal dynamics of signaling activity in live neurons. Although the usefulness of fluorescence resonance energy transfer is well recognized, there are many difficulties in applying it, particularly when imaging in neuronal micro-compartments in light-scattering brain tissue. Fluorescence resonance energy transfer has been imaged using several techniques including intensity-based methods, fluorescence lifetime imaging and fluorescence anisotropy imaging. These methods have different advantages and disadvantages, and thus are suitable in different applications.

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